【特别推荐】康宁MITO+Serum Extender，你值得拥有！

在无血清或近似无血清的条件下培养哺乳动物细胞可大幅改善对细胞增殖、分化的维持和控制。引入确定的培养基添加剂可减少血清的使用，为细胞生长和分化的机制研究提供很有价值的关键视角，也促进了商业化生产，特别是细胞来源的生物制品的纯化。减少培养基中血清的用量也可降低血清批次间差异带来的负面影响。

 

康宁MITO+Serum Extender是浓缩的成分限定的细胞培养添加剂，主要成分包括激素、生长因子和其它代谢物，如EGF、转铁蛋白、重组人胰岛素等。适用于低血清或无血清细胞培养体系。

主要特点

 

 

• 用于无血清/低血清培养体系下的培养基添加剂

• 由经过0.2um滤膜过滤的溶液冻干制成

• 以冻干粉末的形式于2°C~8°C保存，请勿冷冻。（溶解后请分装存储于-20°C，建议在6个月内使用完。避免反复冻融。）

• 细菌、真菌和支原体的检测结果均为阴性

• 在不同的应用中添加量不同。（1ml贮存液可用于配制1L培养基，其中血清含量可比通常使用的减少50%至80%。在一些应用中，MITO+也可以1ml贮存液配制1L无血清培养基。）

• 在使用MITO+的应用中，应将胰蛋白酶的处理时间和胰蛋白酶的浓度保持在最低水平

   

使用康宁MITO+Serum Extender作为培养基组分支持细胞培养的相关文献

小鼠三阴性乳腺癌细胞系Py230（如果没有 MITO，Py230无法保持其细胞特性）

Gibby, K., You, WK., Kadoya, K.et al. Early vascular deficits are correlated with delayed mammary tumorigenesis in the MMTV-PyMT transgenic mouse following genetic ablation of the NG2 proteoglycan. Breast Cancer Res 14, R67 (2012). 

Vito, A., Salem, O., El-Sayes, N. et al. Immune checkpoint blockade in triple negative breast cancer influenced by B cells through myeloid-derived suppressor cells. Commun Biol 4, 859 (2021). 

Excess endocrine growth hormone in acromegaly promotes the aggressiveness and metastasis of triple-negative breast cancer. Kang, Chan Woo et al. iScience, Volume 27, Issue 7, 110137

Balcioglu, O., Gates, B.L., Freeman, D.W. et al. Mcam stabilizes a luminal progenitor-like breast cancer cell state via Ck2 control and Src/Akt/Stat3 attenuation. npj Breast Cancer 10, 80 (2024). 

Abudula, M., Astuti, Y., Raymant, M. et al. Macrophages suppress CD8+T cell cytotoxic function in triple negative breast cancer via VISTA. Br J Cancer 133, 40–51 (2025). 

 

小鼠胃癌细胞YTN2 ，YTN3，YTN 5 和 YTN16

Yamamoto M, Nomura S, Hosoi A, Nagaoka K, Iino T, Yasuda T, Saito T, Matsushita H, Uchida E, Seto Y, Goldenring JR, Kakimi K, Tatematsu M, Tsukamoto T. Established gastric cancer cell lines transplantable into C57BL/6 mice show fibroblast growth factor receptor 4 promotion of tumor growth. Cancer Sci. 2018 May;109(5):1480-1492. doi: 10.1111/cas.13569. Epub 2018 Apr 15. PMID: 29532565; PMCID: PMC5980194.

Ziman, B., Yang, Q., Zheng, Y. et al. Epigenomic analyses identify FOXM1 as a key regulator of anti-tumor immune response in esophageal adenocarcinoma. Cell Death Dis 15, 152 (2024). 

Streptococcus anginosus promotes gastric inflammation, atrophy, and tumorigenesis in mice.Fu, Kaili et al. Cell, Volume 187, Issue 4, 882 - 896.e17

Kimura, T., Takagane, K., Itoh, G. et al. Extracellular vesicles of cancer cells induce FOXP3+ fibroblasts and facilitate tumor invasion via the Wnt3-β-catenin pathway. Oncogene 44, 4101–4113 (2025). 

 

星形胶质细胞

Martínez San Segundo P, Terni B and Llobet A (2023) Multivesicular release favors short term synaptic depression in hippocampal autapses. Front. Cell. Neurosci. 17:1057242. doi: 10.3389/fncel.2023.1057242

Lohse, M. et al. (2025). Investigating the Molecular Composition of Neuronal Subcompartments Using Proximity Labeling. In: Soykan, T., Poulopoulos, A. (eds) Synapse Development. Methods in Molecular Biology, vol 2910. Humana, New York, NY. 

 

神经元细胞

Carmi, I., De Battista, M., Maddalena, L. et al. Holographic two-photon activation for synthetic optogenetics. Nat Protoc 14, 864–900 (2019).

Heather A. Dantzler, David D. Kline,Exaggerated potassium current reduction by oxytocin in visceral sensory neurons following chronic intermittent hypoxia, Autonomic Neuroscience, Volume 229,2020,102735,ISSN 1566-0702, 

 

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